It is important to use sterile sample containers even for non-biological experiments. Otherwise bacteria will create a biofilm which can clog the FluidFM cantilever and bias your experiments.
Non-sterile consumables should be sterilized for example by autoclaving them. As an alternative you can properly clean them with ethanol.
A good cleaning protocol for FluidFM experiments is describes by Orane Guillaume-Gentil et al. (2014):
- Clean dishes with 2-propanol.
- Rinse them with filtered ddH2O water.
- Dry them with a nitrogen stream.
- Plasma-treat them for 2 minutes.
- The dish is now ready for FluidFM experiments.
- Guillaume-Gentil, O., Zambelli, T., & Vorholt, J. (2014). Isolation of single mammalian cells from adherent cultures by fluidic force microscopy. Lab on a Chip, 14(2), 402–14. doi:10.1039/c3lc51174j