Video: Example of single cell force spectroscopy (SCFS) with FluidFM
This video is based on a protocol developed at ETH Zürich in the lab of Prof. Julia Vorholt.
Measure & Clean
When working with mammalian cells, they can stick easily to the probe after an adhesion measurement. However, this depends strongly on the used cell type:
Strongly adherent: Fibroblasts
Weakly adherent: HeLa Cells
The probe needs to be cleaned when a cell sticks to it. We recommend the following cleaning solutions:
During cleaning just leave the probe on the AFM but exchange the petridish with one containing the cleaning solution. Dip the probe in the cleaning solution until it is visibly clean. Then dip the probe in water and continue with the next cell.
More details can be found in the following article
Application Note (PDF):