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Single cell force spectroscopy with FluidFM

Video: Example of single cell force spectroscopy (SCFS) with FluidFM

This video is based on a protocol developed at ETH Zürich in the lab of Prof. Julia Vorholt.

Selected Publications

Rapid and Serial Quantification of Adhesion Forces of Yeast and Mammalian Cells, Potthoff et al, PLoS ONE 2012 7(12) doi:10.1371/journal.pone.0052712


Toward arational design of surface textures promoting endothelialization. Potthoff et al. , Nanoletters 2014, doi:10.1021/nl404739 

 

Measure & Clean

When working with mammalian cells, they can stick easily to the probe after an adhesion measurement. However, this depends strongly on the used cell type: 

Strongly adherent: Fibroblasts

Weakly adherent: HeLa Cells

The probe needs to be cleaned when a cell sticks to it. We recommend the following cleaning solutions:

During cleaning just leave the probe on the AFM but exchange the petridish with one containing the cleaning solution. Dip the probe in the cleaning solution until it is visibly clean. Then dip the probe in water and continue with the next cell.

More details can be found in the following article

 

Application Note (PDF): 

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Comments

  • Avatar
    Tamas

    Would it be possible to include a washing protocol after each adhesion measurement, so it could be fully automatic? Now the measured cell always sticks on the probe, and it needs to be washed afterwards, so we cannot measure more cells one after the other, in an automatic way.

  • Avatar
    Pablo Doerig

    Dear Tamas
    That is a very good suggestion, we will take this into account for future updates of ARYA. The washing itself can already be automated as you know, yet it is not a part of the adhesion workflow. We will think of a way to combine these two to allow automated washing and measurement.
    Best regards, Pablo

    Edited by Pablo Doerig
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