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Sterilizing sample containers

It is important to use sterile sample containers even for non-biological experiments. Otherwise bacteria will create a biofilm which can clog the FluidFM cantilever and bias your experiments.

Non-sterile consumables should be sterilized for example by autoclaving them. As an alternative you can properly clean them with ethanol.

A good cleaning protocol for FluidFM experiments is describes by Orane Guillaume-Gentil et al. (2014):

  1. Clean dishes with 2-propanol.
  2. Rinse them with filtered ddH2O water.
  3. Dry them with a nitrogen stream.
  4. Plasma-treat them for 2 minutes.
  5. The dish is now ready for FluidFM experiments.

References

  • Guillaume-Gentil, O., Zambelli, T., & Vorholt, J. (2014). Isolation of single mammalian cells from adherent cultures by fluidic force microscopy. Lab on a Chip, 14(2), 402–14. doi:10.1039/c3lc51174j

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