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Single cell force spectroscopy with FluidFM

Video: Example of single cell force spectroscopy (SCFS) with FluidFM

This video is based on a protocol developed at ETH Zürich in the lab of Prof. Julia Vorholt.

Selected Publications

Rapid and Serial Quantification of Adhesion Forces of Yeast and Mammalian Cells, Potthoff et al, PLoS ONE 2012 7(12) doi:10.1371/journal.pone.0052712


Toward arational design of surface textures promoting endothelialization. Potthoff et al. , Nanoletters 2014, doi:10.1021/nl404739 

 

Measure & Clean

When working with mammalian cells, they can stick easily to the probe after an adhesion measurement. However, this depends strongly on the used cell type: 

Strongly adherent: Fibroblasts

Weakly adherent: HeLa Cells

The probe needs to be cleaned when a cell sticks to it. We recommend the following cleaning solutions:

During cleaning just leave the probe on the AFM but exchange the petridish with one containing the cleaning solution. Dip the probe in the cleaning solution until it is visibly clean. Then dip the probe in water and continue with the next cell.

More details can be found in the following article

 

Application Note (PDF): 

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