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FAQ

FluidFM experiments

Which Petri-dishes do you recommend?

If you do not have any special requirements for your setup, we recommend to use WillCo glass bottom dishes for any FluidFM experiment.

They offer a very large accessible area and allow the use of high numerical aperture objectives.

Recommended dish: GWSB - 5040

 

Can I exchange the liquid in the probe?

The dead volume is at least 0.5 ul. With typical flowrates of 0.2 nL/s (FluidFM micro pipette @ 1 bar, water in water) replacing a liquid would take about an hour. Ten times more for FluidFM nano pipettes.

This exchange of the liquid also is likely to introduce cross-contamination for the subsequent liquid.

The complete exchange of the liquid is therefore not recommended.

You can however apply frontloading to use several liquids in a row. Here, suck in a small amount of liquid into the probe from the front, only to dispense it again shortly after.

The probe volume is around 30 pL, make sure you suck in less liquid than that. Still OK are for example 10 s @ 10 mbar vacuum (20 pL total). Diffusion and a hydrostatic offset pressure make it however hard to contain the liquid in the probe longer than a few minutes.

FluidFM Probes


Why can I not fill the probe?


Any liquid you use should be filtered before use.
Please check the troubleshooting in the probe section for detailed steps on how to deal wit this problem.

Are there other probe designs available?


If you want a custom probe design we can offer you two options:

  • If you have access to a focused ion beam facility (FIB) we can supply you with FluidFM rapid prototyping probes, with a hollow channel and a pyramidal tip. You then have the maximum flexibility to shape the pyramidal tip and its opening.
  • If you do not have access to a FIB facility we can perform the prototyping for you on request. 
  • If you need large quantities of a custom FluidFM probes, we can develop it for you on request. You will have to cover the initial development costs and receive >100 probes of your chosen design. The new FluidFM probe type will afterwards stay available for the regular price. 


Pressure controller

Where does the click noise come from?

The pressure controller typically produces a clicking noise during pressure regulation. It corresponds to a special valve movement in the controller unit, which allows for a quick pressure adjustment.


Why does the pressure controller not reach the desired pressure/vacuum?


It is likely that there is a leak somewhere between the pressure controller and the FluidFM. Please check if the tube does not have any tears or holes and that it is tightly connected.

Where can I download the pressure controller standalone software?


The software is available under the following link http://www.cytosurge.ch/download/pctrl/

Cytosurge Operator Software 

Where are the log-files located?

If you encounter an error and want to report it to us, it helps to also include the log-files. They are located in 

C:\Users\"Current User"\AppData\Local\Cytosurge\Logs\log.txt

The AppData folder is hidden. It can be accessed by typing the address into the explorer or by enabling hidden files and folders

 

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